Examining the Relationship between Oral Microbiome Dysbiosis and Systemic Inflammation: A Clinical Laboratory Investigation in a Tertiary Care facility

Ali E. Alblowi; Ahad N. Albulayhid; Amjaad A. Mohammed; Norah M. Alfaifi

doi:10.5281/zenodo.15222872

Examining the Relationship between Oral Microbiome Dysbiosis and Systemic Inflammation: A Clinical Laboratory Investigation in a Tertiary Care facility

Author(s)	Ali E. Alblowi, Ahad N. Albulayhid, Amjaad A. Mohammed, Norah M. Alfaifi
Country	Saudi Arabia
Abstract	Background: Dysbiosis of oral microbiome may be associated with chronic inflammation, although clinically integrated studies within multidisciplinary laboratories are scarce in developed countries. In tercial care settings. Objectives: Examine the association between oral microbiome dysbiosis and systemic inflammation in patients at a tertiary care hospital. Methods: The study was a cross sectional comprising 120 participants, 60 with periodontitis and 60 age and sex matched healthy controls. Clinical periodontal examinations were performed, accompanied sample collection of unstimulated salivary and blood. 16S rRNA sequencing was conducted for oral microbiome analysis alongside serum inflammatory markers, including CRP, IL-6, and TNF-alpha which were analyzed using ELISA. Results were presented with descriptive statistics and differences between means were tested using t-test. Results: Periodontitis subjects were significantly low diverse in microbial diversity with a Shannon diversity index of 3.2 ± 0.6 with an increase in bacterial taxa, including Porphyromonas gingivalis and Fusobacterium nucleatum (p<0.001) while control subjects had an index of 4.7 ± 0.5. Moreover, periodontitis patients exhibited significantly high markers of systemic inflammation. Levels were strongly positively correlated with pathogenic taxa and inflammatory markers with CRP levels yielding r=0.68 p <0.001. Analysis of beta diversity confirmed significant clustering of microbes within groups p=0.002. Conclusion: This work illustrates the link of oral microbiome dysbiosis to heightened systemic inflammation. Results emphasize the role of oral health care in reducing the burden of inflammation and systemically integrated approaches for early diagnosis and intervention in tertiary care models.
Field	Medical / Pharmacy
Published In	Volume 14, Issue 2, April-June 2023
Published On	2023-05-12
Cite This	Examining the Relationship between Oral Microbiome Dysbiosis and Systemic Inflammation: A Clinical Laboratory Investigation in a Tertiary Care facility - Ali E. Alblowi, Ahad N. Albulayhid, Amjaad A. Mohammed, Norah M. Alfaifi - IJSAT Volume 14, Issue 2, April-June 2023. DOI 10.5281/zenodo.15222872
DOI	https://doi.org/10.5281/zenodo.15222872
Short DOI	https://doi.org/g9fk2q

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Examining the Relationship between Oral Microbiome Dysbiosis and Systemic Inflammation: A Clinical Laboratory Investigation in a Tertiary Care facility

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