Isolation Characterization and Optimization of Growth Conditions for Azotobacter spp.

Author(s)	Anshad A
Country	India
Abstract	ABSTRACT: The role of genus Azotobacter in increasing the growth and health of plants is very important. The present study deals with the optimization of growth condition for Azotobacter species. Four colonies were isolated from soil and Azotobacter were found in them. The medium M2 allowed for the highest growth of Azotobacter (0.015mglml) and 0.3mg/ml. Optimum temperature ,pH and incubation period for growth of Azotobacter were 25 degree Celsius,9 and 48 hrs., respectively .By enhancing the growing medium with various carbon and nitrogen sources ,the growth speciality of Azotobacter was investigated. The most effective concentration for Azotobacter were 2.5% mannitol (as a C- source) and 2%(NH4)2 SO4 (as an N-source) 24hrs old inoculum at a level of 1% was found best for the growth of Azotobacter .The bio fertilization activity was studied on maize plant in pot experiment and it was found that plant inoculated with Azotobacter gave better growth as compared to control plants.
Keywords	Azotobacter, Maize plant, pH
Field	Biology > Agriculture / Botany
Published In	Volume 16, Issue 1, January-March 2025
Published On	2025-02-24
Cite This	Isolation Characterization and Optimization of Growth Conditions for Azotobacter spp. - Anshad A - IJSAT Volume 16, Issue 1, January-March 2025. DOI 10.71097/IJSAT.v16.i1.2068
DOI	https://doi.org/10.71097/IJSAT.v16.i1.2068
Short DOI	https://doi.org/g85944

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